Yet again, tyres on the vehicle belonging to Little Footsteps Nursery on Columbus Ravine were damaged with what police believe was a screwdriver.
The attack, reported last Wednesday, is the latest in a string of incidents of damage to the nursery's minibus.
Natalie Lamb, from the nursery, said every time the minibus needs to be repaired, youngsters have to miss trips out.
"It's just a shame because we are an outdoors nursery and we use the bus to take the children out on their adventures.
"It's the children who are missing out."
Police are appealing for anyone who has information about the damage to call them on 101, quoting reference number 12200223824.
Information can also be passed on anonymously via Crimestoppers on 0800 555111.