Ayton down after battling defeat
Ian Jenkinson twice put Duchess ahead, only for Adrian Kwiatkowski and Chris Milburn to make it 2-2 at the break.
Duchess wrapped up the win with goals from JP Watson (2) and sub Ian Laing, Tyler Beck replying for Ayton.
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Hide AdPaul Oldroyd shone for Ayton with Luke Midgley the Duchess star man.
In Division One, Edgehill eased to a 7-0 home win against a weakened Seamer.
The Edgehill scorers were Danny Glendinning (2), Mikey Barker, Tyler Richardson, Jackson Jowett, keeper Liam Giles and a 20-yard volley from Dan Jones.
Itis Itis Rovers maintained their impressive finish to the third division season when beating Goal Sports 4-2, ending the promoted side's unbeaten home record.
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Hide AdTom Thornton put Rovers ahead after good work from Neil Forsyth, then a Niall Gibb penalty and Thornton's second from a Dave Young assist made it three.
Tom Wilde and Niall Prentice brought it back to 3-2, but Forsyth lashed home a loose ball to seal Rovers' victory.
Thornton was named man of the match for the winning side.
Fishburn Park and Sherburn played out a thriller, which ended 3-3.
Sherburn moved two goals clear courtesy of a fine solo effort from Sam Rackham and a Paul Mills penalty.
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Hide AdAlex Wiggins pulled one back to keep Park in the game just before half-time.
Harry Walmsley looked to have put Sherburn on the path to the points with a 30-yard free-kick, but the understrength side faded in the closing stages and Park grabbed a point.
Wiggins tapped home his second and Theo Clarke's drive put Fishburn on terms.
Sixteen-year-old keeper Kieran Purvis impressed for Fishburn, as did winger Jack Kipling.
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Hide AdSleights Reserves powered to an 11-0 win at Eastway, with Rob Ingham, Ben Watson and Spencer Hewison hitting a brace each.
Ryan Roe, Taylor Humble, Carl Harris, Mike Dent and player-boss Rob Winspear, in his final game in charge, also notched.
Hunmanby United Reserves beat Scalby Reserves 3-1.
Nick Turley slotted Scalby ahead after a great ball from Tommy Day, but United levelled before the break.
Hunmanby added two more goals in the closing stages to seal the win