This was Day's second spell with the club, having returned last September after his first stint at the Seadogs when Steve Kittrick snapped him up after he impressed in grassroots football in the Bridlington area.
The club released a statement on their website, which said: "Scarborough Athletic would like to express thanks to Jake Day who has left the club following the end of the season.
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"Jake leaves with our best wishes and he will forever be part of the squad that got Boro promoted to the National League North. A young fan favourite, Day always gave his all on and off the pitch.
"Further news on the playing front will be released when appropriate as Jono shapes his squad for the National League North."
Day said on social media: "What a season it’s been! What a club! A promotion and a cup But unfortunately it’s been a short but sweet stay at Scarborough. Thank you to the gaffer, all the staff and all my teammates for making this season possible!
"I’d also like to thank all the fans for the unbelievable love and support they’ve shown me from the moment I came to the club! I wish you all the best for the future and hopefully I’ll see you all again let’s see what’s next
"As for now it’s time to rest, recover and spend some time with my family."